Tryptophan Pathway Abnormalities in a Murine Model of Hereditary Glaucoma

Background: It has been shown that a possible pathogenetic mechanism of neurodegeneration in the mouse model glaucoma (DBA/2J) may be an alteration kynurenic acid (KYNA) retina. This study aimed to verify hypothesis alterations tryptophan (TRP) metabolism DBA/2J mice is not limited Methods: Samples retinal tissue and serum were collected from (6 10 months old) control C57Bl/6 same age. The concentration TRP, KYNA, kynurenine (KYN), 3-hydroxykynurenine (3OH-K) was measured by HPLC. activity indoleamine 2,3-dioxygenase (IDO) also determined as KYN/TRP ratio. Results: L-KYN, 3OH-K significantly lower retinas than mice. higher older both strains. Serum KYNA concentrations age-matched controls. However, IDO did differ between compared groups Conclusions: Alterations TRP pathway seem retina murine hereditary glaucoma.